Identifying crossover locations in Arabidopsis thaliana wild type and taf4b Col × Bur F2 populations using genotyping-by-sequencing. Identifying crossover locations in Arabidopsis thaliana wild type and taf4b Col × Bur F2 populations using genotyping-by-sequencing

Genomic DNA from 180 Col/Bur F2 individuals, and 186 taf4b/Bur F2 individuals, was extracted by CTAB and used to generate sequencing libraries as previously described (Ziolkowski et al, 2017 Genes & Dev). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bethesda); Yelina et al, 2015 Genes & Dev).