Methylomic profiling of melanoma tissues from patients prior to immune checkpoint blockade to identify predictors of response or resistance

Genome wide DNA methylation profiling of formalin-fixed paraffin-embedded (FFPE) melanoma tissue samples from patients prior to treatment with anti-PD1 immune checkpoint inhibitor therapy. The MethylationEPIC v1.0 BeadChip array was used to obtain DNA methylation profiles across approximately 850,000 CpGs in bisulfite treated DNAs extracted from formalin-fixed paraffin embedded melanoma tissues prior to treatment with anti-PD1 immune checkpoint inhibitor therapy. Bisulphite converted DNA from the melanoma FFPE tissue samples were hybridized to the Illumina Infinium Human 850K MethylationEPIC v1.0 BeadChip array. Samples were collected prior to immune checkpoint blockade therapy.