Role of RccR in the transcriptional control of primary carbon metabolism in Pseudomonas

To characterise the RccR regulon, we performed a ChIP-seq assay using a polyclonal RccR antiserum on SBW25 WT/?rccR strains grown in minimal pyruvate and glycerol media. We were able to identify 8 RccR binding sites from this experiment including one in the rccR promoter region itself. The peaks identified in our RccR ChIP-seq assays corresponded to strongly enriched regions relative to the respective ?rccR controls. All 8 peaks were localized in intergenic regions, upstream of one or more genes.