List of target genes.

*These organisms may be found in a healthy airway on rare occasions. When detected in pneumonic patients, they are very likely to be the causative pathogen. We therefore list them as non-commensal organisms.# F: forward primer, B: backward primer, D: detection probe. Each primer set was tested against the DNA panel and was confirmed to provide specific amplification only for its target sequence. The panel contains DNA from human, from 65 organisms, and from 2 organisms that harbor drug resistance-related genes; the organisms include Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Staphylococcus aureus, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Chlamydophila psittaci, Legionella pneumophilia, Mycobacterium tuberculosis, Mycobacterium intracellulare, Mycobacterium avium, Mycobacterium kansasii, Nocardia asteroids, Pneumocystis jiroveci, Acinetobacter baumannii, Anaerococcus hydrogenalis, Aspergillus flavus, Aspergillus fumigates, Aspergillus niger, Bacteroides caccae, Bacteroides fragilis, Bacteroides thetaiotaomicron, Candida albicans, Candida parapsilosis, Clostridium perfringens, Clostridium ramosum, Corynebacterium spp., Coxiella burnetii, Cryptococcus neoformans, Eikenella corrodens, Enterobacter aerogenes, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Haemophilus haemolyticus, Haemophilus parainfluenzae, Klebsiella oxytoca, Legionella bozemanii, Legionella micdadei, Mycobacterium gordonae, Neisseria meningitides, Peptostreotococcus anaerobius, Porphyromonas asaccharolytica, Prevotella bivia, Prevotella intermedia, Proteus mirabilis, Serratia marcescens, Staphylococcus auricularis, Staphylococcus epidermidis, Staphylococcus simulans, Streptococcus anginosus, Streptococcus constellatus, Streptococcus intermedius, Streptococcus mitis, Streptococcus pyogenes, Streptococcus uberis, Trichosporon asahii, and Trichosporon mucoides; drug resistance-related genes include IMP and mecA.