The DIGE Experimental Design for Minimal Labeling with Cy Dyes used in this study.
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A total of 90 µg of labeled proteins were loaded on each gel for 2D electrophoresis; Tear sample from early, intermediate and late stage keratitis patients were used. Labeling, construction of pooled standards are described under materials and methods. 250 pmoles of dye per 30 µg protein was used.
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2015-12-02
