MALAT-1 is a long mammalian non-coding transcript of approximately 8500 nucleotides. It is localized to nuclear speckles despite its mRNA-like characteristics, which usually result in the export of t
Additional files 1: Supplementary Figs. 1–9. Fig. S1: Proof of concept of APEX-seq in sIOs. a, Left: Scheme of the COX4 construct (also known as MITO-V5-APEX2) used as a bait to capture RNAs localizin
We performed cryosectioning of oocytes along the animal-vegetal axis (first developmental axis, section A (first animal) to section E (last vegetal), followed by RNA-Seq to determine the localization