Sam68 regulation of alternative splicing in the developing cerebellum

We uncover a Sam68-dependent splicing program during cerebellar development. These events direct proper isoform expression of the genes required to guarantee the establishment of the correct spatial/temporal neural circuitry. The dysregulation in Sam68 null mice leads to functional defects in adult neurons Examination of 3 different RNA from P1, P10 and P30 wild type cerebella of mouse, and 2 from P1 and P10 Sam68 null cerebella本研究揭示了小脑发育过程中依赖于Sam68的可变剪接调控程序。此类调控事件可指导基因的正确异构体表达，从而确保时空精准的神经环路得以建立。Sam68敲除小鼠体内的调控紊乱会导致成年神经元出现功能缺陷。本研究对小鼠P1、P10及P30龄野生型小脑组织的3份不同RNA样本，以及P1、P10龄Sam68敲除小鼠小脑组织的2份RNA样本开展了检测。