Single-cell RNA sequencing of liver nonparenchymal cells from murine model of endotoxemia

Liver has a crucial role in the regulation of immune defense in systemic infections. During endotoxemia, the liver transits from an immune-tolerant towards an immune-active state and forms the first line of defense against invading microorganisms. the role of liver nonparenchymal cells in endotoxemia remains unintelligible. To characterize the liver nonparenchymal cells in endotoxemia liver, we performed single-cell RNA sequencing of liver nonparenchymal cells from healthy C57BL/6J mice and murine model of endotoxemia at early or late stage. The single-cell RNA-seq analyses revealed the heterogeneity of liver nonparenchymal cells in endotoxemia liver. Single-cell RNA sequencing of liver nonparenchymal cells from healthy C57BL/6J mice (n=6, eight weeks-age) using MGISEQ-2000. Single-cell RNA sequencing of liver parenchymal cells from murine model of endotoxemia at 3-hour time point (n=6, eight weeks-age) using MGISEQ-2000. Single-cell RNA sequencing of liver parenchymal cells from murine model of endotoxemia at 12-hour time point (n=6, eight weeks-age) using MGISEQ-2000.