PI3K Signaling Specifies Proximal-Distal Fate by Driving a Developmental Gene Regulatory Network in SOX9+ Lung Progenitors [RNA-seq]

In order to understand the epigenetic mechanisms directing development and differentiation of SOX9+ lung epithelial progenitor cells (EPCs), paired RNA-Seq and ATAC-Seq was performed on SOX9+ EPCs sorted using flow cytometry from a Sox9-GFP reporter mouse. Overall design: GFP+ positive lung epithelium was isolated by flow cytometry by selecting GFP+, CD326+, CD31-, CD45-, 7-AAD-negative cells into Trizol. Total RNA was isolated using RNEasy Micro Kits, and poly A RNA was sequenced as described below.