NO DIFFERENCES IN MICROBIOME CHANGES BETWEEN ANTI-ADHESIVE AND ANTIBACTERIAL INGREDIENTS IN TOOTHPASTES DURING PERIODONTAL THERAPY

Aim: This subgroup analysis of a 12-week prospective, randomized, double-blind, two-center trial aimed to evaluate if two different toothpaste-formulations can differentially modulate the dental microbiome.Materials and Methods: 41 mild to moderate periodontitis patients used as an adjunct to periodontal treatment either a toothpaste with anti-adhesive carbonate/hydroxyapatite or with anti-microbial/anti-adhesive amine-fluoride/stannous-fluoride during 12 weeks period. Plaque samples from buccal/lingual, interproximal and subgingival sites were taken at baseline, 4 weeks after oral hygiene phase, and 8 weeks after periodontal therapy. Samples were analyzed with Illumina Miseq 16S rDNA sequencing. The differences and changes on community-level (alpha and beta diversity) and on the level of single agglomerated ribosomal sequence variants (aRSV) were calculated with Analysis of Covariance (ANCOVA) and Likelihood Ratio Test (LRT).Results: Interproximal and subgingival sites harbored predominately Fusobacterium and Prevotella associated with periodontitis, whereas buccal/lingual sites harbored mainly Streptococcus and Veillonella associated with periodontal health. Alpha and beta diversity did not change noticeably differently between both toothpaste-groups (p>0.05, ANCOVA). Furthermore, no aRSV showed a noticeably different change between the tested toothpastes during periodontal therapy (padj.>0.05, LRT).Conclusions: The use of a toothpaste containing anti-adhesive carbonate/hydroxyapatite did not induce statistically different changes on microbial composition compared to an anti-microbial/anti-adhesive amine fluoride/stannous fluoride.