Genome-wide CRISPR loss-of-function screen for α-amanitin

The “death cap”, Amanita phalloides, is the world’s most poisonous mushroom, responsible for 90% of mushroom-related fatalities. The most fatal component of the death cap is α-amanitin(AMA). Despite its lethal effect, the exact mechanisms of how α-amanitin poisons humans remain unclear, leading to no specific antidote available for treatment. Here, we performed a genome-wide CRISPR loss-of-function screen to identify genes and pathways involved in AMA cytotoxicity. The mutant cell pools were treated by AMA for 7 days and the surviving cells were recovered and harvested for genomic DNA analysis. Two replicates for control group and AMA group.