Moe Mahjoub, Tim Stearns (2010) CIL:10060, Mus musculus, tracheal epithelial cell. CIL. Dataset

Mouse tracheal epithelia cells (MTEC) were grown at air-liquid interface to induce differentiation, centriole amplification and ciliogenesis. Cells were fixed using ice-cold methanol for 10 min. Samples were stained using antibodies against acetylated tubulin to mark cilia (green), the centriole protein Cep120 to mark basal bodies (red) and cell-cell junctions using ZO-1 (blue). Secondary antibodies were Alexa 488 for acetylated tubulin, Alexa 594 for Cep120, and Cy5 for ZO-1. Images were captured using Openlab 4.0.4 software controlling an Axiovert 200M microscope (Carl Zeiss, Inc.) and a 100X 1.3 NA objective.

小鼠气管上皮细胞（MTEC）在气-液界面培养以诱导分化、中心粒扩增及纤毛发生。细胞经冰冷的甲醇固定10分钟。样品使用针对乙酰化微管蛋白的抗体染色以标记纤毛（绿色），使用中心粒蛋白Cep120标记基座（红色），以及使用ZO-1标记细胞间连接（蓝色）。二抗为Alexa 488（乙酰化微管蛋白）、Alexa 594（Cep120）和Cy5（ZO-1）。图像通过Openlab 4.0.4软件控制Axiovert 200M显微镜（卡尔·蔡司公司）及100倍1.3数值孔径物镜进行捕捉。