Figure 5: Fractal assemblies capture and release greater amounts of cargo compared to globular assemblies

<b>Figure 5: Fractal assemblies capture and release greater amounts of cargo compared to globular assemblies. </b>Scheme for the envisioned reversible capture of cargo proteins for the (<b>A</b>) fractal and (<b>B</b>) globular structures. The red stars denote an example cargo protein (antibody). (<b>C</b>) % protein capture was measured for 3:2 fractal (assemblies obtained with 3 AtzA-pY: 2 AtzC-SH2), 3:2 GS linker (globular assemblies obtained at the same stoichiometry with fusion proteins containing long GS-rich linkers), and 3:1 fractal (assemblies obtained with 3 AtzA-pY: 1 AtzC-SH2). The 3:2 fractal captured more IgG antibody (red bars) and GFP-SH2 (green barss), and degraded more substrate TCP (purple bar; reflecting the higher capture efficiency of enzyme DhaA-Sh2) than the 3:2 GS linker assemblies. In addition, 3:2 fractal released more captured antibody compared to the 3:2 GS linker when incubated with YopH phosphatase (blue bars). (<b>D</b>, <b>E</b>) Confocal fluorescence microscopy images of the 3-component assembly with GFP-SH2 showing the topology of incorporation of GFP-SH2 in fractal and (<b>F-G</b>) the incorporation of GFP-SH2 into the globular assemblies. (<b>H-I</b>) the IgG antibody Alexa Fluor 568 incorporation into the fractal assembly and (<b>J-K</b>) the incorporation into the globular assembly.<b></b>