Physiological mechanisms of shell microstructural regulation in Japanese pearl oyster under hypoxic stress: Insights into molluscan resilience against environmental changes

Investigating the anomalous expression of nacre, one of the most common microstructures of the Mollusca, as an environmental archive and its genomic background provides novel insights into sclerochronology and biomineralization. In this study, we aimed to elucidate the physiological responses of the Japanese pearl oyster, Pinctada fucata, under hypoxia, focusing on shell microstructure plasticity. We conducted RNA-seq analysis in specimens from different depths with different oxygen concentrations and observed changes in microstructures. We found differences in shell microstructure and distinct gene expression profiles between hypoxia and normoxia. Several shell matrix protein genes were downregulated under hypoxia, suggesting their role in shell formation. We estimated the dissolved oxygen threshold affecting the physiological resistance of P. fucata to microstructural alterations. These results indicate that molluscan shell microstructures are under genetic controls in response to env..., , , # Dataset: Physiological mechanisms of shell microstructural regulation in Japanese pearl oyster under hypoxic stress

Physiological mechanisms of shell microstructural regulation in Japanese pearl oyster under hypoxic stress: Insights into molluscan resilience against environmental changes, Marine Pollution Bulletin, 2026, DOI: [https://doi.org/10.1016/j.marpolbul.2025.119082](https://doi.org/10.1016/j.marpolbul.2025.119082)

Dryad dataset DOI: [https://doi.org/10.5061/dryad.pnvx0k6zn](https://doi.org/10.5061/dryad.pnvx0k6zn)

## Overview

This dataset contains differential expression (DE) results from RNA-seq of Pinctada fucata under normoxia vs hypoxia, and functional annotation outputs.

Each DEG table is provided per tissue.

### Experimental groups (important for interpreting m.value sign)

- G1: normoxia

- G2: hypoxia

Note: In TCC, M = log2(G2) − log2(G1).

## File inventory

All files are comma-separated values (CSV) with a header row, UTF-8 encoding.

### 1) DEG_list_of_adduc...,