Mutations in cdon and boc affect trunk neural crest cell migration and slow-twitch muscle development in zebrafish

Bulk RNA-seq of isolated slow-twtich muscle and neural crest cells from 24 hours post fertilization zebrafish. Bulk RNA-seq data from FAC sorted slow-twitch muscle and neural crest cells isolated from trunks of 24 hours post fertilization zebrafish embryos. Trunks of embryos were manually dissected and cells were disassociated using Accumax. Embryos were doubly transgenic for tg(sox10:mRFP) transgene that labels neural crest cells and tgPAC(prdm1a:eGFP) transgene that labels slow-twitch muscle and muscle pioneer cells. Neural crest cells and slow-twitch muscle cells were isolated by FACS on a MoFlow XDP100, prepared for stranded Illumina sequencing, and sequenced on a NovaSeq600 (PE 150bp). Gene expression summaries were produced using featurecounts (subread package) and DESeq2.