Alternative splicing of ALDOA determines tamoxifen resistance in breast cancer

Via analyzing the landscape of AS events in primary and relapse breast tumors from patients receiving tamoxifen-based therapy, it was revealed that exon skipping (ES) of exon 7.2 in 5'UTR of ALDOA pre-mRNA is associated with tamoxifen resistance. Therefore, RNA Sequencing is performed for MCF7-TAMR cells infected with ctr or ALDOA shRNAs to identify the molecular mechanism of ALDOA in mediating TAM resistance in breast cancer cells. Overall design: We established MCF7-TAMR cell lines in which ALDOA was knockdowned by shRNA targeting ALDOA.