Viability of SH-SY5Y neuroblastoma cells treated with ATRA (dose-response) alone or in the presence of AA-signalling inhibitors.

*ATRA alone: Viability at 15 µM.SH-SY5Y cells were pre-treated for 2 h with inhibitor (or vehicle control) at the concentrations specified (left column) prior to the addition of ATRA and viability was measured after 24 h incubation with ATRA in the presence of inhibitor using XTT assays. The % viability of the cells treated with the specified dose of inhibitor alone (middle right column) is given alongside the IC50 of ATRA and inhibitor together (right column). Unless otherwise stated, the IC50 was calculated from data where 0% viability resulted from the maximal ATRA concentration. For viability experiments, there were 6 technical replicates for each dose-response curve and each dose-response curve was performed three times. Results are mean %±SEM.