RNA-seq, computational analysis of the secretome of brown algae. Laminariales

In order to reveal the repertory of the brown algal secretome and its involvement in the evolution of Laminariales, we conducted de novo transcriptome sequencing using seven laminarialean species (Agarum clathratum, Alaria crassifolia, Aureophycus aleuticus, Costaria costata, Pseudochorda nagaii, Saccharina angustata and Undaria pinatifida) largely covering the laminarialean families. The transcripts encoding the secretome of the laminarialean species were searched utilizing the secretome of Ectocarpus and Saccharina japonica predicted from their published complete genome data. We focused on the functional elaboration of the secretome in the laminarialean lineage linked to the alteration of the domain architecture and gene family abundance.