B cell analyses for PD-1- and PD-L1-deficient humans and PD-1 KO mice

We tested the hypothesis that PD-1:PD-L1 signaling is essential for optimal B-cell memory and antibody responses by studying the recently described PD-1-deficient (Ogishi et al. Nat. Med. 2021) and PD-L1-deficient (Johnson, Ogishi, Domingo-Vila et al. JEM 2024) patients, as well as PD-1 KO mice.
This repository contains processed data and analyses for (matched figures in the corresponding paper):
- Single-cell trajectory analysis of B cells of the PD-1- and PD-L1-deficient patients (Fig. 2).
- BCR repertoire analysis of sorted naive and memory B cells of the PD-1-deficient patient and whole-blood leukocytes of the PD-L1-deficient patients (Fig. S2).
- Bulk RNASeq of in vitro stimulated PBMCs of the PD-1- and PD-L1-deficient patients (Fig. 4).
- Bulk RNASeq of sorted naive and memory B cells of the PD-1-deficient patient and some disease controls (Fig. S2, 2, and 6).
- Bulk RNASeq of sorted naive B cells from healthy donors stimulated in the presence of PD-1 or PD-L1-neutralizing antibodies (Fig. S5).
- Bulk RNASeq of sorted naive and memory B cells from WT and PD1 KO mice (Fig. 3).