Genome-wide expression profiling of human prostate stromal cells in response to multiple chemotherapeutic agents

The DNA damage secretory program (DDSP) provoked by the side effects of genotoxic therapeutics results in senescence associated secretory phenotype (SASP) and fuels advanced pathologies particularly cancer resistance. However, the complete mechanism underlying SASP development in the tumor microenvironment (TME) remains unclear. We treated PSC27, a primary normal human prostate stromal cell line, with two distinct groups of chemotherapeutic drugs that are either genotoxic or not, and determined the key molecules and their associated pathways responsible for development of a typical SASP phenotype under DNA damage conditions. ArrayStar human LncRNA Microarray v3.0 (8 x 60K, ArrayStar, Rockville, MD) was used to profile expression of PSC27 cells treated with various chemotherapeutic agents to induce cell damage in vitro. Total RNA was isolated and amplified prior to hybridization in a one-color experiment. There were totally seven samples analyzed, each composed of three biological replicates, including controls and experimental groups.