PGC Retention Times

Porous graphitised carbon-liquid chromatography (PGC-LC) has been proven to be a powerful technique for the analysis and characterisation of complex mixtures of isomeric and isobaric glycan structures. Here we evaluate the elution behaviour of N-glycans on PGC-LC and thereby provide the potential of using chromatographic separation properties, together with mass spectrometry (MS) fragmentation, to determine glycan structure assignments more easily.多孔石墨化碳液相色谱法（Porous graphitised carbon-liquid chromatography, PGC-LC）已被证实为分析、表征异构与同量异位聚糖结构复杂混合物的强有力技术。本研究考察了N-聚糖在PGC-LC上的洗脱行为，借此证实了结合色谱分离特性与质谱（MS）碎裂技术，可更便捷地实现聚糖结构的精准归属。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于多孔石墨化碳液相色谱（PGC-LC）技术，该技术被证明能有效分析复杂异构糖链结构。通过评估N-糖链在PGC-LC上的洗脱行为，结合质谱碎裂技术，可更便捷地确定糖链结构。