Transcriptomic characterization of CRISPR/Cas9-edited THP1 cells to test the effect of disrupting the 3q23 genomic region containing TB-risk associated variants marking a putative monocyte-specific enhancer

We applied CRISPR/Cas9 technology to introduce insertions/deletions around the top associated variant rs73226617. Among 23 single-cell sorted and expanded clones that had unchanged 3q23 genomic region with the risk locus (i.e. wildtype), or harbored unique edits and deletions around rs73226617. Low input RNA-seq was performed on all 23 sorted and grown THP1 clonal cell lines that had unchanged 3q23 genomic region with the TB-risk variants or harbored unique edits and deletions