Time course for bladder UCC development in UPII-SV40Tag mice

We have identified genes that are differentially expressed between the bladders of UPII-SV40Tag mice and their age-matched wild-type littermates at 3, 6, 20, and 30 weeks of age. These are ages that correspond to premalignant, carcinoma in situ, and early-stage and later stage invasive UCC, respectively Analysis of the microarray data sets has revealed ~1,900 unique genes differentially expressed (>=3-fold difference at one or more time points) between wild-type and UPII-SV40Tag urothelium during the time course of tumor development. RNA from the urothelium of duplicate UPII-SV40Tag hemizygous and age-matched wild type littermate mice was isolated at 3, 6, 20, and 30 weeks of age and gene expression profiles were obtained.我们已鉴定出3、6、20和30周龄时，UPII-SV40Tag小鼠膀胱与其同龄野生型同窝仔鼠膀胱之间的差异表达基因。这些年龄阶段分别对应癌前病变、原位癌（carcinoma in situ）以及早期和晚期侵袭性尿路上皮癌（UCC）。对微阵列（microarray）数据集的分析显示，在肿瘤发生的时间进程中，野生型与UPII-SV40Tag尿路上皮（urothelium）之间存在约1900个独特的差异表达基因（在一个或多个时间点的表达差异≥3倍）。本研究分别于3、6、20和30周龄，从两份平行的UPII-SV40Tag半合子（hemizygous）小鼠及同龄野生型同窝仔鼠的尿路上皮中分离RNA，并获取了基因表达谱。