The nerve roots avulsion induced time-specific expression patterns of lncRNA and mRNA occurred before the motoneurons loss of the injured spinal segments

In this study, we study the expression pattern of the lncRNAs in rat’s spinal cord of at 3 days and 14 days after the unilateral BPRA. By comparing the expression patterns of mRNAs and lncRNAs, ipsilateral vs contralateral, on the 3rd and the 14th day, in the same spinal segments using microarray analysis. We identified 55 lncRNAs, which were significantly altered at the 3rd day after BPRA. Two weeks after BPRA, 70 lncRNAs which were significantly altered. Among these DE lncRNAs, 17 were consistently over-regulated at 3- and 14- days after injury while 5 lncRNAs were consistently down-regulated. At 3-days after injury, 399 mRNAs were significantly differentially expressed while two weeks after BPRA, 445 were downregulated. Among these mRNAs, 198 were consistently over-regulated, and 15 mRNAs were consistently under-regulated.本研究聚焦单侧BPRA术后3天及14天的大鼠脊髓组织中长链非编码RNA（long non-coding RNAs，lncRNAs）的表达模式。通过基因芯片分析，对比同一脊髓节段内术后第3天与第14天同侧与对侧的mRNA及长链非编码RNA表达谱。本研究共鉴定出55个在BPRA术后第3天发生显著表达变化的长链非编码RNA；术后2周则有70个长链非编码RNA出现显著表达差异。在上述差异表达长链非编码RNA（differentially expressed lncRNAs，DE lncRNAs）中，17个在损伤后3天及14天均持续上调，另有5个持续下调。损伤后第3天，共有399个mRNA发生显著差异表达；BPRA术后2周则有445个mRNA表达下调。在这些差异表达mRNA中，198个持续上调，仅15个持续下调。