Whole transcriptome sequencing using Huh7 cells transfected with Dengue virus serotype 4 NS1 expressing plasmid.

To study the role of Dengue virus serotype 4 NS1 in modulation of host cell transcriptome we performed gene expression profiling using data obtained from RNA- sequencing of total RNA from 3 different samples: Huh7 cells (no transfection), Huh7 cells transfected with pTracer-SV40 empty vector and Huh7 cells transfected with pTracer-SV40 encoding dengue virus serotype 4 NS1 (pDENV4 NS1). After 24 hours of transfection, cells were lysed and total RNA was purified. RNA samples were proccessed for whole transcriptome sequencing using Illumina Platform, followed by comparative gene expression analysis.