Treatment of microglia with NCM and tuftsin induced the release of immunomodulatory factors.

To characterize the factors released from microglia under EAE-like conditions, microglia were treated for 10 hours with NCM isolated from neurons undergoing excitotoxic injury in the presence or absence of tuftsin. Concentrated conditioned media were analyzed by multidimensional chromatography and tandem mass spectrometry. Partial list of the secreted proteins is shown. Spectral counts (MS/MS) of proteins identified from the media of microglia treated with NCM alone (MgCM) or a combination of NCM and 100 µg/ml of tuftsin (MgCM+Tuf) for 10 hours are listed in the respected columns. Ratios derived from spectral counts were used to predict the trend of differential expression.