Metformin induces chromosome reorganization and changes in gene expression in normal human fibroblasts

We report the effects of metformin treatment on the transcriptome of normal human dermal fibroblasts isolated from foreskin (designated 2DD). We sequenced mRNA from 2 replicates of proliferative (PRO) and 2DD cells treated with two concentrations of metformin (0.5mM and 1.0 mM;5 days exposure). Comparative analyses with PRO transcripts a baseline indicate that fibroblsts exposed to different concentration of metformin result in differnetial transcript profiles. Overall design: Examination of mRNAs from proliferative and metformin treated (0.5mM and 1.0 mM for 5 days) 2DD normal human dermal/foreskin fibroblasts.