Single-cell CRISPR Screen for HIV Host Dependency Factors Involved in Latency Reversal

Perturb-seq reveals intrinsic and extrinsic biological factors that regulate gene functions in laten HIV expression. We used a Jurkat HIV cell model that we previously established for pooled CRISPR screening, where cells stably expresses Cas9 and are latently infected with HIV-GFP viral vectors. We performed Perturb-seq experiments on three different conditions, including stimulated Jurkat (by PMA/I) followed by GFP expression sorting (GFP+ or GFP-), or unstimulated cells.