Complete genome sequencing and assembly of NTHi 3655 (NTHi 3655KR)

Nontypeable Haemophilus influenzae 3655 (NTHi 3655; also known as NTHi 3655KR in our lab collection), was isolated from the middle ear effusion of a child in Missouri (USA) with acute otitis media. This strain is non-capsulated hence is noted as “nontypeable”. This strain has been widely used in the our study of host-pathogen interaction of NTHi; the strain uses host complement regulators and ECM proteins for serum resistance and enhanced adherence, respectively. The genome sequence of this strain was initially sequenced, assembled and deposited as 23 contigs in GenBank. Here we re-sequence the strain for a complete whole genome sequence with full annotation. The strain sequence will be used for our genomic/transcriptomic/proteomics comparative analysis.