Measured interstitial volumes (Vi) in naïve and anti-VEGF-administered mice.

Note: Literature values are listed for comparison. Nude mice (n = 5) were naive or administered a single, intravenous bolus dose (10 mg/kg) of the cross-species anti-VEGF antibody [12], B20-4.1, 24 h prior to assay. All values are reported in µL/g. Note that the assay method can greatly influence measured Vi values, especially in probe clearance organs.a[14].b[5].c[23].dTetramethylammonium (TMA+) method; corrected for a reported 9.4 µL/g blood volume [14].eReflects inability of 111In-DTPA to cross the blood-brain barrier.fNon-physiologically relevant measurement due to renal clearance of 111In-DTPA.gExtrapolated from rat; sodium (extracellular marker) content determined in a flame photometer [22].hEstimated on the basis of values for similar tissues [5].iExtrapolated from rat; 51Cr-EDTA method [5], [23].