Supplementary table 3

Polar metabolite dataPolar metabolite profiling data were generated for a mouse KGC pancreatic tumor line expressing doxycycline-inducible constitutively-active mutant GNAS (GNASR201C) growing in 2D culture in the presence of doxycycline; Dox(+) or absence of doxycycline; Dox(-). The cells were harvested as 5 biological replicates and flash-frozen. The polar metabolites were extracted with 40:40:20 acetonitrile/methanol/water and replicates are denoted as Dox(+)-1, Dox(+)-2, Dox(+)-3, Dox(+)-4, Dox(+)-5 and Dox(-)-1, Dox(-)-2, Dox(-)-3, Dox(-)-4, Dox(-)-5. Polar metabolites were analyzed by QQQ-LC/MS/MSas described in the reference1.The ion counts were normalized to the total cell number. Metabolites were quantified by integrating the area under the curve, and then normalized to internal standard values. 1) Louie, S. M.et al.GSTP1 Is a Driver of Triple-Negative Breast Cancer Cell Metabolism and Pathogenicity. Cell Chem Biol23, 567-578, doi:10.1016/j.chembiol.2016.03.017 (2016).