Transcriptional profiling of mammary gland and spleen from Mycoplasma agalactiae infected sheep. Transcriptional profiling of mammary gland and spleen from Mycoplasma agalactiae infected sheep

We used RNA-seq to investigate gene expression changes in sheep mammary gland and spleen tissue after experimental infection with Mycoplasma agalactiae (strain PG2). Sheep (3 per each group) were given an intra-mammary infection with 10^9 cfu infectious organisms or PBS as control. The animals were euthanized 15 days post infection to obtain the samples. Two replicates of mammary gland and spleen tissue per animal were used for Illumina RNA-sequencing.