Data associated with "Correlative Raman and immunofluorescence imaging reveals different protein abundance between stress granules induced by oxidative damage"

<b>Figures</b>Figure S1: Representative IF images of cells treated under increasing NaAsO₂ or H₂O₂ concentrations at the indicated durationsFigure S2: Representative IF images of cells under each treatment labeled with anti-G3BP1Figure 1A: Relative reactive oxygen species generated by each stress treatment measured by DCFH-DA assayFigure 1B and S3: Western blot of Nrf2 protein levels in stressed cells and corresponding Ponceau-S stainFigure 2A, 2B, and 2C: Representative IF images of cells under each treatment labeled with anti-G3BP1, anti-eIF4G, and anti-TDP-43 antibodies taken at confocal and super resolutionFigure 2D: Number of cells per 100 counted which have a SG-phenotype determined by IF stainingFigure 2E: Average number of SGs produced per SG-positive cell determined by IF stainingFigure 3A: Excitation and emissions spectra of Alexa405 and Alexa647 dyes taken on a fluorometerFigure 3B: Representative multi-layer IF image of cells treated with sodium arsenite for 1 h and labeled with anti-G3BP1 and anti-Figure 3C: Averaged Raman spectrum of a cell focused on the C–H stretching region after background subtractionFigure 3D–G: Raw RSI map data used to generate maps of lipid, protein, and first moment analysisFigure 3H: Averaged Raman spectra of outlined puncta and cytosol identified by first moment analyses in Fig. 3GFigure S4: Integrated area under CH₃ stretch of Raman spectrum taken at indicated <i>Z</i>-position through a single point in a cellFigure 4: Raw RSI map data used to generate first moment analyses and correlative widefield fluorescence images of anti-G3BP1Figure 5: Averaged Raman spectra of outlined puncta and adjacent cytosol in Fig. 4Figure 6, S5, S6, and S7: Correlative, multi-layer IF images and raw RSI map data used to generate <i>z</i>-stacks