Thermodynamic analysis of the d3'EBS1*/IBS1* equilibrium by different methods.
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The experimental error was estimated by bootstrapping and accounts for 99.7% of the variability observed (3σboba, “68–95–99.7 rule” [66]). Association constants Ka were calculated from normalized cumulated FRET histograms or dwell times under the assumption that [IBS] = [IBS1*]tot as described in the Supplementary Information S1 (Eqs. S1 and S2).
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2015-12-02
