Agaricus bisporus var. burnettii strain:H119_p4 Genome sequencing and assembly. Agaricus bisporus var. burnettii strain:H119_p4

Agaricus bisporus, the most cultivated edible mushroom worldwide, is represented mainly by the two subspecies var. bisporus and var. burnettii. Var. bisporus has a secondarily homothallic life cycle with recombination restricted to chromosome ends, while var. burnettii is heterothallic with recombination seemingly equally distributed over the chromosomes. To better understand the relationship between genomic make-up and different life styles, we have de novo sequenced a burnettii homokaryon and updated the published genomes of var. bisporus. The genomes were assembled into telomere-to-telomere chromosomes and a consistent set of gene predictions was generated. The genomes of both subspecies were largely co-linear, and especially the chromosome ends differed in gene model content between the two subspecies. A single large cluster of repeats was found on each chromosome at the same respective position in all strains, harbouring nearly 50% of all repeats and likely representing centromeres. Repeats were all heavily methylated. Finally, a mapping population of var. burnettii confirmed an even distribution of crossovers in meiosis, contrasting the recombination landscape of var. bisporus. The new findings using the exceptionally complete and well annotated genomes of this basidiomycete demonstrate the importance for further unravelling of the genetic components underlying the different life cycles.