Gene expression profiling using extracted RNA from MLL-AF9/Mn1wt and MLL-AF9/Mn1null cells

We evaluated the function of Meningioma 1 (MN1), a cofactor of HOXA9 and MEIS1, in murine MLL-r leukemia by CRISPR-Cas9 mediated deletion of MN1. MN1 was required for in vivo leukemogenicity of MLL-r murine and human AML cells. Loss of MN1 inhibited cell cycle and proliferation, promoted apoptosis and induced differentiation of MLL-r cells. Expression analysis and chromatin immunoprecipitation with sequencing demonstrated that MN1 primarily maintains active transcription of HOXA9 and HOXA10, which are critical downstream genes of MLL, and their target genes like BCL2, MCL1 and Survivin. CRISPR-Cas9 mediated deletion of MN1