BMV RNA replication is not affected in the absence of HSE1 or VPS36.
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https://figshare.com/articles/dataset/_Host_ESCRT_Proteins_Are_Required_for_Bromovirus_RNA_Replication_Compartment_Assembly_and_Function_/1327958
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Total RNA extracts were obtained from wt, hse1Δ, or vps36Δ deletion strains expressing 1a, 2apol, and RNA3 and accumulation of negative-strand RNA3 and positive-strand subgenomic RNA4 was measured by Northern blotting. Equal loading was verified by probing for 18S ribosomal RNA. Values represent the mean of four independent experiments, with each condition tested in triplicate in each experiment. A representative blot is shown. All samples were compared to the wt control using a two tailed student t-test. ns: not significant. (EPS)
创建时间:
2015-12-03



