Transcriptome profiling of muscle stem cell subpopulations based on differential expression of Myf5

To identify genes and pathways differentially expressed in Myf5 subpopulations of muscle stem cells Myf5 subpopulations (Myf5High and Myf5Low) of muscle stem cells were isolated from hindlimb muscles of Pax7/Myf5 reporter mice by fluorescence-activated cell sorting (FACS). Myf5High and Myf5Low cells were defined as two Pax7-positive subpopulations at opposite ends of the Myf5-E2Crimson fluorescence spectrum. In total, 4 different conditions were analyzed: male Myf5High, male Myf5Low, female Myf5High, and female Myf5Low. We performed two independent replicates per condition, each replicate consisting in a pool of 4 mice.