Data for: Developmental origins of Parkinson’s disease risk: perinatal exposure to the organochlorine pesticide dieldrin leads to sex-specific DNA modifications in critical neurodevelopmental pathways in the mouse midbrain

Epidemiological studies show that exposure to the organochlorine pesticide dieldrin is associated with increased risk of Parkinson’s disease (PD). Animal studies support a link between developmental dieldrin exposure and increased neuronal susceptibility in the α-synuclein preformed fibril (α-syn PFF) and MPTP models in adult male C57BL/6 mice. In a previous study, we showed that developmental dieldrin exposure was associated with sex-specific changes in DNA modifications within genes related to dopaminergic neuron development and maintenance at 12 weeks of age. Here, we used capture hybridization-sequencing with custom baits to interrogate DNA modifications across the entire genetic loci of the previously identified genes at multiple time points – birth, 6 weeks, 12 weeks, and 36 weeks old. We identified largely sex-specific dieldrin-induced changes in DNA modifications at each time point that annotated to pathways important for neurodevelopment, potentially related to critical steps i..., Animals: Adult female and male C57BL/6 mice (RRID:MGI:2159769) were purchased from Jackson Laboratory (Bar Harbor, Maine). Seven-week-old female mice were allowed to habituate after arrival for 1 week prior to beginning the developmental dieldrin exposure. Male mice were 11 weeks old upon arrival and were also allowed 1 week to habituate prior to mating. Mice were maintained on a 12-hour:12-hour reverse light/dark cycle. Mice were housed in Thoren ventilated caging systems with automatic water and 1/8-inch Bed-O-Cobs bedding with Enviro-Dri for enrichment. Food and water were available ad libitum. Mice were maintained on Teklad 8940 rodent diet (Envigo). After arrival, females were separated and individually housed during dieldrin dosing, except during the mating phase. After birth, F1 pups were group-housed by sex, with no more than 5 animals housed in each cage. No singly housed F1 animals were used in this study; as animals aged, all cages included a “buddy” littermate to ensure that..., , # Data for: Developmental origins of Parkinson’s disease risk: perinatal exposure to the organochlorine pesticide dieldrin leads to sex-specific DNA modifications in critical neurodevelopmental pathways in the mouse midbrain

[https://doi.org/10.5061/dryad.ffbg79d30](https://doi.org/10.5061/dryad.ffbg79d30)

This dataset contains all code and output from the differential methylation analysis, as well as raw data and supporting analysis files for mass spectrometry.

## Description of the data and file structure

**Supplementary Files**

* Supplementary_File_1_primary_targets.bed: This bed file contains targeted regions and has the following columns: chromosome number, start coordinate, end coordinate, gene name.

- Supplementary_File_2_capture_targets.bed: This bed file contains designed baits and has the following columns: chromosome number, start coordinate, end coordinate, gene name.
- Supplementary_File_3\_ Consolidated_DMCs.csv: This csv file contains the merged results of the cross...