Neural Stem Cell Extracellular Vesicle-Treated Microglia and Transcriptional Response

Extracellular vesicles (EVs) were isolated from primary cultures of neural stem cells (NSCs) and used to treat microglia. EVs were isolated by serial centrifugation from primary NSCs cultured from the subventricular zone of CD1 mice. Cultured microglia were then subjected to control or EV treatments and RNA isolated by Trizol extraction. Library preparation was performed with an NEBNext Ultra RNA Library Preparation Kit according to the manufacturer’s protocol (New England Biolabs). 2 x 150 base pair reads were generated at a depth of 56-84 million reads per sample on an Illumina HiSeq 4000.