Peripheral Blood Mononuclear Cell Profiling using Fluidigm's Peripheral Blood Panel

Peripheral Blood Mononuclear Cells from three independent donors were profiled using Fluidigm's Peripheral Blood panel (http://www.dvssciences.com/products/pdf/TDS_201304.pdf). The dataset was used to illustrate a new visualization method for high dimensional data.
Conclusion:
Radviz, a new multidimensional visualization method, was used to review and compare the samples. In one of the donors an increase in the number of CD8-positive T cells was visualized and confirmed using manual gating. Radviz was also used to compare specific populations across donors.
Notes:
The experiment was performed using 3 independent blood samples taken from healthy donors. Instrument performance was monitored using labelled beads mixed with cells (1/10). Dead cells and debris were manually gated.