Gastrointestinal microbiome study in patients infected with Mycobacterium tuberculosis. Microbiome in TB and LTBI patients

BackgroundBy 16S rRNA gene analysis, we had been observed the Firmicutes to Bacteroidetes (F/B) ratio and Bifidobacteriaceae in gut microbiome was related to the active tuberculosis (TB) or latent TB infection (LTBI) among HIV-uninfected individuals. We further measured biomarkers including CD3+, CD4+, CD8+ T cells, white blood cell distribution in peripheral blood, interleukin-1 beta (IL-1B), IL-4, IL-6, IL-10, invariant nature killer T (iNKT), and interferon-gamma releasing assay to determine whether the gut microbiome was associated with innate immunity after MTB infection in HIV-uninfected individuals.