Dataset for the article "Structural and Functional Insights into Oxidized and Hydrogenated HPHT Nanodiamonds for Cortisol Immunosensor Applications".

Dataset for the article "Structural and Functional Insights into Oxidized and Hydrogenated HPHT Nanodiamonds for Cortisol Immunosensor Applications

Chakavak Esmaeili1, Štěpán Stehlík2, Martin Krejci3, Bohuslav Rezek1

1 Faculty of Electrical Engineering, CTU in Prague, Technicka 2, 16627 Prague, Czech Republic

2 Institute of Physics of the Czech Academy of Sciences, Cukrovarnická 10, 162 00 Prague, Czech Republic

3 FHNW University of Applied Sciences and Arts Northwestern Switzerland School of Engineering Klosterzelgstrasse 2CH-5210 Windisch

Fig 1A. SEM images of (a) the carbon bare electrode  (b) before and (c) after rinsing  of 3 µg HPHT-OFig 1B. SEM image of (a) the carbon bare electrode  (b) before and (c) after rinsing  of 3 µg HPHT-HFig 1C. SEM micrographs of (a) bare carbon electrode, (b) SPE/HPHT-O, (c) SPE/HPHT-O/APTES, (d) SPE/HPHT-O/APTES/EDC NHS, (e) SPE/HPHT-O/APTES/EDC NHS/Ab, (f) SPE/HPHT-O/APTES/EDC NHS/Ab/BSA, (g) SPE/HPHT-O/APTES/EDC NHS/Ab/BSA/Ag (with original magnification 2.00KX (up) and 15.00KX (down) at 5 kV)Fig 2. The preparation and immobilization step of the cortisol fabrication based on electrochemical immunosensor Fig 3. Functionalization step. a) CV and b) DPV results of (1) carbon bare electrode, (2) electrode/HPHT-O, (3) electrode/HPHT-O/APTES, (4) electrode/HPHT-O/APTES/EDC NHS, at pH 7.0, containing 250 mM KCl and 5 mM K3[Fe(CN)6] and 5 mM K4[Fe(CN)6], Scan rate 0.1Vs−1Fig 4. Sensing steps. a) CV and b) DPV results for (4) electrode/HPHT-O/APTES/EDC NHS, (5) electrode/HPHT-O/APTES/EDC NHS/Ab, (6) electrode/HPHT-O/APTES/EDC NHS/Ab/BSA, and (7) electrode/HPHT-O/APTES/EDC NHS/Ab/BSA/Ag in the presence of 1 ng/mL cortisol at pH 7.0, in a solution containing 250 mM KCl, 5 mM K₃[Fe(CN)₆], and 5 mM K₄[Fe(CN)₆], with a scan rate of 0.1 V/sFig 5. a) CV and b) DPV results for (1) carbon bare electrode, (2) electrode/HPHT-O, (3) electrode/HPHT-O/APTES, (4) electrode/HPHT-O/APTES/Ab, (5) electrode/HPHT-O/APTES/Ab/BSA, and (6) electrode/HPHT-O/APTES /Ab/BSA/Ag in the presence of 1 ng/mL cortisol at pH 7.0, in a solution containing 250 mM KCl, 5 mM K₃[Fe(CN)₆], and 5 mM K₄[Fe(CN)₆], with a scan rate of 0.1 V/sFig 6. a) CV and b) DPV results for (1) carbon bare electrode, (2) electrode/HPHT-O, (3) electrode/HPHT-O/EDC NHS, (4) electrode/HPHT-O/ EDC NHS /Ab, (5) electrode/HPHT-O/ EDC NHS /Ab/BSA, and (6) electrode/HPHT-O/ EDC NHS /Ab/BSA/Ag in the presence of 1 ng/mL cortisol at pH 7.0, in a solution containing 250 mM KCl, 5 mM K₃[Fe(CN)₆], and 5 mM K₄[Fe(CN)₆], with a scan rate of 0.1 V/sFig 7. a) CV and b) DPV results of (1) carbon bare electrode, (2) electrode/HPHT-H, (3) electrode/HPHT-H/APTES, (4) electrode/HPHT-H/APTES/EDC NHS, (5) electrode/HPHT-H/APTES/EDC NHS/Ab, (6) electrode/HPHT-H/APTES/EDC NHS/Ab/BSA, and (7) electrode/HPHT-H/APTES/EDC NHS/Ab/BSA/Ag in the presence of 1 ng/mL cortisol at pH 7.0, in a solution containing 250 mM KCl, 5 mM K₃[Fe(CN)₆], and 5 mM K₄[Fe(CN)₆], with a scan rate of 0.1 V/sFig 8. Variation of the immunosensor response against the cortisol concentrations in the range of 1.0 ng to 0.5 ng/mL at pH 7.0, in a solution containing 250 mM KCl, 5 mM K₃[Fe(CN)₆], and 5 mM K₄[Fe(CN)₆], with a scan rate of 0.1 V/sFig 9. Interference study involving progesterone (2430 pg/ml), β-oestradiol (32 pg/ml), cortisone (28.6 nM), and corticosterone (3.94 nM) with respect to cortisol (1 ng/mL)

Fig S1.  Optical images obtained for (a) before and (b) after rinsing of 3.0µg HPHT-HFig S2. Optical images obtained for (a) bare carbon electrode, (b) HPHT-O, (c) HPHT-O/APTES, (d) HPHT-O/APTES/EDC NHS, (e) HPHT-O/APTES/EDC NHS /Ab, (f) HPHT-O/APTES/EDC NHS /Ab/BSA, (g) HPHT-O/APTES/EDC NHS /Ab/BSA/AgFig S3. a) CV and b) DPV results of (1) carbon bare electrode, (2) electrode/HPHT-H, (3) electrode/HPHT-H/APTES, (4) electrode/HPHT-H/APTES/Ab, (5) electrode/HPHT-H/APTES/ Ab/BSA, and (6) electrode/HPHT-H/APTES/Ab/BSA/Ag in the presence of 1 ng/mL cortisol at pH 7.0, in a solution containing 250 mM KCl, 5 mM K₃[Fe(CN)₆], and 5 mM K₄[Fe(CN)₆], with a scan rate of 0.1 V/SFig S4. a) CV and b) DPV results of (1) carbon bare electrode, (2) electrode/HPHT-H, (3) electrode/HPHT-H/EDC NHS, (4) electrode/HPHT-H/EDC NHS/Ab, (5) electrode/HPHT-H/EDC NHS/ Ab/BSA, and (6) electrode/HPHT-H/EDC NHS/Ab/BSA/Ag in the presence of 1 ng/mL cortisol at pH 7.0, in a solution containing 250 mM KCl, 5 mM K₃[Fe(CN)₆], and 5 mM K₄[Fe(CN)₆], with a scan rate of 0.1 V/SFig S5. a) CV and b) DPV results of (1) carbon bare electrode, (2) electrode/APTES, (3) electrode/APTES/EDC NHS, (4) electrode/APTES/EDC NHS/Ab, (5) electrode/APTES/EDC NHS/Ab /BSA, and (6) electrode/APTES/EDC NHS/Ab/Ag in the presence of 1 ng/mL cortisol at pH 7.0, in a solution containing 250 mM KCl, 5 mM K₃[Fe(CN)₆], and 5 mM K₄[Fe(CN)₆], with a scan rate of 0.1 V/S

Table 1. Summary of the DPV electrochemical performance of the HPHT-O modified electrode using APTES and EDC-NHS as crosslinkers in cortisol immunosensor detectionTable 2. Summary of the DPV electrochemical performance of the HPHT-O modified electrode utilizing APTES without EDC-NHS as a crosslinker in cortisol immunosensor detection.Table 3. Summary of the DPV electrochemical performance of the HPHT-O modified electrode utilizing EDC-NHS without APTES as a crosslinker in cortisol immunosensor detectionTable 4. Determination of cortisol in artificial human saliva.