Establishment of hTERT immortalized mouse cardiosphere derived cell line. Establishment of hTERT immortalized mouse cardiosphere derived cell line

The analysis showed differences of transcriptomes between primary CDCs and immortalized CDCs as well as revealed TGF-β inhibitor-SB431542 treatment induced expression changes. We identified hTERT immortalization could result the cell proliferation and cell cycle related genes upregulation in iCDC. KEGG results demonstrated that TGF-β inhibitor SB431542 treatment could inhibit cardiomyopathy-related pathway which indicate TGF-β signaling pathway may serve as new target for cardiomyopathy therapy, and iCDC could be used as a heart-related chemicals screening. Overall design: Primary CDCs, iCDCs and SB432542-treated iCDCs mRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq2000.