File S1 - Characterization of the RnfB and RnfG Subunits of the Rnf Complex from the Archaeon Methanosarcina acetivorans

Supporting figures. Figure S1, SDS-PAGE of purified normal RnfG and variant RnfG-T166A from Methanosarcina acetivorans. Figure S2, Sequence alignment of RnfG from Methanosarcina acetivorans with NqrC from Vibrio cholerae. Figure S3, SDS-PAGE of RnfB from Methanosarcina acetivorans. Figure S4, Sequence comparisons of the RnfB subunit from Methanosarcina acetivorans. Figure S5, Membrane topology of RnfA, RnfD and RnfE predicted with the HMMTOP algorithm. (PDF)