Expression of neuronal and glial markers in teratomas grown in 129Sv and SCID/beige mice after injection of ES and differentiated cells.

Teratomas were obtained after injection of ES cells or differentiated (diff.) cells into syngeneic 129Sv mice and immunodeficient SCID/beige mice. Histological sections were stained by immunohistochemistry for the neuronal marker NeuN and the glial marker GFAP. The presence of the markers is indicated semi quantitatively in four categories: − negative, + single positive cells, ++ groups of positive cells, +++ confluent groups of positive cells. Five representative teratomas per group were analyzed.12 of 5 teratomas were negative for this marker.23 of 5 teratomas were negative for this marker.