OsterlundJBC_Figure 2

<b> Figure 2 data </b> <br><br>
Includes: <br>
-Panel B, colocalization data. Pearson's correlation values were exported per cell in Cell Profiler, then minimum intensity thresholds were applied to ensure the cells were stained/expressing the protein(s) of interest. Here is the resulting summary where we exported the median and mode of each set of data. <br><br>
-Panel C- F, FLIM-FRET data acquired for one biological replicate acquired where date acquired, Cell line, and mCerulean3-donor is given within each Excel worksheet. Raw binding curve data was binned by Venus intensity. Here we provide the resulting binned data, and fitting parameters exported from GraphPad prism used to create the binding curves that were displayed in the paper. The remaining biological replicates are uploaded with "OsterlundJBC_SFigure 2".<br><br>
-Panel G, Cell death measured in MCF-7 cells and MCF-7 cells expressing mCerulean3-tagged BCL-2 or BCL-XL. Raw data were binned by Venus intensity. Here we provide the resulting binned data from 3 biological replicates, and the fitting parameters exported from GraphPad prism for curves displayed in the paper. <br><br>
-Panel H, Cell death measured in HEK293T cells. AnnexinV positivity is a marker of Cell death. Here we provide the mean cell death measured in Venus positive cells from 3 biological replicates.