IFN lambda modulates dendritic cells to facilitate T cell immunity during influenza virus infection

Type I and type III interferon (IFN) are important in regulating responses to viral infection. Type I IFN signaling is know to contribute to dendritic cell (DC) function, but the contribution of type III IFN remains unknown. To determine changes in migratory dendritic cell gene expression during influenza A virus (IAV) in the presence or absence of type I or type III (IFN) receptors, C57Bl/6 (WT) mice, mice lacking the type I IFN receptor (Ifnar-/-), and mice lacking the type III IFN receptor (Ifnlr-/-) were infected intranasally with 40 plaque forming units (PFU) IAV H1N1 strain A/PR/8/34 or mock infected. On day 4, lung-draining lymph nodes (dLN) were harvested, conventional DC were isolated, and mRNA sequencing (mRNAseq) was performed. Overall design: DCs were enriched from dLN of WT, Ifnar-/-, and Ifnlr-/- mice on day 4 following intranasal mock or infection with 40 PFU A/PR/8/34. These enriched DCs were then FACS sorted to isolate conventional DC (lineage-/MHCII+/CD11b-/CD11c+). cDNA was amplified and mRNA sequencing was performed.