BCR and apoptosis responses in CLL and healthy B cells.

Percentage of B cells from CLL and healthy samples that undergo H2O2-mediated phosphorylation of intracellular signaling molecules. Values are taken from the 2D cytometry plots (Figures S3(A) and (B), S4(B) and (C) and data not shown). Mean and standard deviations are shown for each CLL and healthy sample. CLL samples were segregated into Group 1 and Group 2 based their H2O2-mediated p-Stat5 response which was statistically significant (p-value 1.6×10–4) between the two groups. There was statistical significance for all other signaling molecules between each group (p-values within the range of 3.8×10–5–9×10–3). The exception was H2O2-mediated p-Akt signaling for which the p-value was 0.5. Apoptosis responses to in vitro F-Ara-A exposure are shown as+for proficient and – for refractory according to the criteria described in the Data Analysis section on page 11. The percentage of cells that are positive for cleaved caspase 3 and cleaved PARP at 48 hours after background subtraction are in parentheses.