Next Generation Sequencing of Wild-Type C57BL/6J Mouse Cardiac Polyadenylated RNA

Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. We have made widespread use of Illumina sequencing technologies for RNA quantitation in several publications involving mouse hearts, dating from 2010, and wish to share both high-quality raw sequencing data and data processed to quantitate mRNA abundances from wild-type mice, male and female, at a variety of ages (see our FVB/NJ data submission). These data will provide a resource for investigators using microarrays to understand the concentration of transcripts of interest relative to other cardiac RNAs, and will permit deeper interpretation of previous microarray studies. 4 cardiac polyA+-RNA profiles of 12 week-old C57BL/6J wild type (WT) mice were generated on Illumina HiSeq 2000 instruments.研究目的：下一代测序（Next-generation sequencing, NGS）可实现RNA丰度定量，并能在组织与细胞内对比RNA丰度，这是既往微阵列（microarray）技术无法达成的。自2010年起，我们在多项针对小鼠心脏的研究中广泛应用Illumina测序技术开展RNA定量工作，现计划共享两类数据：一是高质量的原始测序数据，二是经处理后得到的、来自不同周龄野生型（wild-type, WT）雌雄小鼠的mRNA丰度定量数据（详见我们的FVB/NJ数据集提交内容）。此类数据可为使用微阵列技术的研究者提供参考资源，帮助其明确目标转录本相较于其他心脏RNA的表达浓度，同时也能为既往微阵列研究提供更深入的解读依据。本数据集包含4份12周龄C57BL/6J野生型小鼠的心脏聚腺苷酸化RNA（polyA+ RNA）测序谱，所有测序工作均在Illumina HiSeq 2000测序仪上完成。